|Played by:||Jawa Man66|
Dr. Harleen Quinzel was a psychiatrist interning at Arkham Asylum, having been fascinated in studying The Joker. During their sessions, Quinzel fell madly in love with the Joker, breaking him out of Arkham several times and having her psychiatric license revoked. She would then re-invent herself as Harley Quinn, sidekick and lover of the Joker. Besides the Joker, Harley Quinn also works with Poison Ivy, with whom she is close friends. She is a skilled gymnast as well as having psychiatric knowledge. She refers to the Joker as "Mistah J" or her pet name "Puddin'" rather than by his title.
- Ability: Underestimated (Automatically gets one hit on any male character, including Batman)
- Weakness: Mistah J! (If The Joker and/or Poison Ivy are defeated in a fight, you will be unable to attack again until after both attacks. If only one is defeated, you only have to wait for that character to attack again)